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Pregled bibliografske jedinice broj: 151998

Synthesis and antitumor activity of 5-bromo-1-mesyluracil


Glavaš-Obrovac, Ljubica; Karner, Ivan; Pavlak, Maja; Radačić, Marko; Kašnar-Šamprec, Jelena; Žinić, Biserka
Synthesis and antitumor activity of 5-bromo-1-mesyluracil // XVI International Roundtable International Society of Nucleosides, Nucleotides and Nucleic Acid
Minneapolis, 2004. (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
Synthesis and antitumor activity of 5-bromo-1-mesyluracil

Autori
Glavaš-Obrovac, Ljubica ; Karner, Ivan ; Pavlak, Maja ; Radačić, Marko ; Kašnar-Šamprec, Jelena ; Žinić, Biserka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
XVI International Roundtable International Society of Nucleosides, Nucleotides and Nucleic Acid

Mjesto i datum
Minneapolis, SAD, 12-16.09.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Synthesis; antitumor activity; 5-bromo-1-mesyluracil; in vitro; in vivo

Sažetak
We have prepared novel sulfonylcyclourea derivatives by attachment of sulfonyl fragment on N-1 of pyrimidine bases. The compounds showed potent growth inhibitory activity against human tumor cell lines in vitro, at concentrations of 10-5-10-8 M, and some of them showed the ability to induce apoptosis in treated tumor cells. The purpose of this study was to elucidate the effects of 5-bromo-1-(methanesulfonyl)uracil (BMsU) on the biosynthetic activity of tumor cells' enzymes involved in DNA, RNA and protein synthesis, and in de novo and salvage pyrimidine and purine syntheses. Investigations were performed in vitro on human colon carcinoma (CaCo2) and cervix carcinoma cells (HeLa). BMsU displayed inhibitory effects on DNA and RNA synthesis in CaCo2 and HeLa cells, after 24-hours of treatment. De novo biosynthesis of pyrimidine and purine was also affected in both treated tumor cell lines. Antitumor activity of BMsU is closely associated with its inhibitory activity on enzymes which play important role in the metabolism of tumor cells. The in vivo antitumor activity of BMsU was also investigated. The model used in investigations was a mouse anaplastic mammary carcinoma transplanted into the thigh of the right leg of CBA mice. Significant reduction in tumor growth time was achieved with BMsU administrated in dose of 50 mg/kg.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA


Projekt / tema
0098053
0098145
0127111

Ustanove
Institut "Ruđer Bošković", Zagreb,
Učiteljski fakultet, Zagreb