engleski

Prevention of ochratoxin A by zeolites

Mycotoxins contamination of food and feed is an important health problem, both in humans and animals, almost allover the world. Ochratoxin A (OTA) is nephrotoxic fungal metabolite known to cause nephropathy in farming animals and it is probably involved in the aethiology of human endemic nephropathy (EN). The different protective methods are focused on the removal of mycotoxins from contaminated foodstuffs or to neutralize their toxic activity. Klioptiolits and especially zeolits have been successfully used in agricultural production as very potent absorbents. Commercially available product "Megamin pulver" a zeolite prepared by special tribomechanical procedure was tested in vitro as a possible OTA adsorbent. The OTA absorption by tribomechanically processed zeolit was tested under different pH values (pH 2, pH 4, pH 7.5), simulating conditions in certain parts of digestive system. The presence of OTA was determined by use of HPLC (with detection limit of 0.2 ng/ml). In all pH conditions absorptive possibilities of Megamin were observed, however the highest absorptive properties were noticed at pH 2, suggesting its best potential absorptive effect for OTA in the stomach.

ochratoxin A; zeolits; HPLC

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