engleski

Identification of Plasma Low Density Lipoprotein Subclasses by a New Electrophoresis Method

Human LDL are a heterogenous collection of particles which vary in density, size and lipid and protein composition. Recent investigations have shown that increased proportion of small, dense LDL particles is an important coronary artery disease risk marker. We develop a simple electrophoresis method for separation of LDL particles using a nondenaturating gradient (3, 5-5%) polyacrylamide gel.Using this method, we studied a group of 150 patients with dyslipoproteinemia and 60 controls. Concentrations of total cholesterol, triglycerides, glucose, HDL- and LDL-cholesterol, Lp(a) and apo B were determined in all samples. We established that an increased proportion of small, dense LDL particles has been associated with elevated triglyceride and low HDL-cholesterol concentrations much more frequently than with elevated total and LDL-cholesterol levels.

LDL subclasses; electrophoresis

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