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Insulin-like and Non-insulin-like Action of Vanadate in Hepatocytes Cultured in Vitro

The aim of this study was to determine possible insulin-mimetic effects of vanadate on amino acid transport, glycogen synthesis and glucose production in hepatocytes cultured in vitro. Vanadate like insulin caused significant 100% increase of amino acid transport. However vanadate produced significant 31% decrease of 14C-glucose incorporation into glycogen, in contrast to insulin which caused 54% increase. Vanadate also caused significant decrease (24%) of basal glucose production but had not effect on glucagon-stimulated glucose production. Vanadate and insulin given together stronger reduced basal (68%) and also effectively prevented glucagon-stimulated effect on glucose production. In hepatocytes cultured in vitro vanadate had insulin-like and non-insulin like action. Our results clearly demonstrated that non-insulin like effects of vanadate could have beneficial influence in therapy of diabetes type 2 causing decrease of glucose production from the liver.

amino acid transport; glucose production; glycogen synthesis; insulin; vanadate

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