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Pregled bibliografske jedinice broj: 147755

HPr kinase/phosphorylase, a Walker motif A-containing bifunctional sensor enzyme controlling catabolite repression in Gram-positive bacteria


Poncet, S.; Mijakovic, I.; Nessler, S.; Gueguen-Chaignon, V.; Chaptal, V.; Galinier, A.; Boel, G.; Maze, A.; Deutscher, J.
HPr kinase/phosphorylase, a Walker motif A-containing bifunctional sensor enzyme controlling catabolite repression in Gram-positive bacteria // Biochimica et Biophysica Acta - Proteins and Proteomics, 1697 (2004), 1-2; 123-135 (međunarodna recenzija, članak, znanstveni)


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Naslov
HPr kinase/phosphorylase, a Walker motif A-containing bifunctional sensor enzyme controlling catabolite repression in Gram-positive bacteria

Autori
Poncet, S. ; Mijakovic, I. ; Nessler, S. ; Gueguen-Chaignon, V. ; Chaptal, V. ; Galinier, A. ; Boel, G. ; Maze, A. ; Deutscher, J.

Izvornik
Biochimica et Biophysica Acta - Proteins and Proteomics (1570-9639) 1697 (2004), 1-2; 123-135

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
protein kinase; P-protein phosphorylase; catabolite repression; inducer exclulsion; Gram-positive bacteria

Sažetak
Carbon catabolite repression (CCR) in Gram-positive bacteria is regulated by the bifunctional enzyme HPr kinase/phosphorylase (HprK/P). This enzyme catalyses the ATP- as well as the pyrophosphate-dependent phosphorylation of Ser-46 in HPr, a phosphocarrier protein of a sugar transport and phosphorylation system. HprK/P also catalyses the pyrophosphate-producing, inorganic phosphate-dependent dephosphorylation (phosphorolysis) of seryl-phosphorylated HPr (P-Ser-HPr). P-Ser-HPr functions as catabolite co-repressor by interacting with the LacI/GalR-type repressor, catabolite control protein A (CcpA), and allowing it to bind to operator sites preceding catabolite-regulated transcription units. HprK/P thus indirectly controls the expression of about 10% of the genes of Gram-positive bacteria. The two antagonistic activities of HprK/P are regulated by intracellular metabolites, which change their concentration in response to the absence or presence of rapidly metabolisable carbon sources (glucose, fructose, etc.) in the growth medium. Biochemical and structural studies revealed that HprK/P exhibits no similarity to eukaryotic protein kinases and that it contains a Walker motif A (or P-loop) as nucleotide binding site. Interestingly, HprK/P has a structural fold resembling that in kinases phosphorylating certain low molecular weight substrates such as nucleosides, nucleotides or oxaloacetate. The structures of the complexes of HprK/P with HPr and P-Ser-HPr have also been determined, which allowed proposing a detailed mechanism for the kinase and phosphorylase functions of HprK/P.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
0098071

Profili:

Avatar Url Ivan Mijaković (autor)


Citiraj ovu publikaciju:

Poncet, S.; Mijakovic, I.; Nessler, S.; Gueguen-Chaignon, V.; Chaptal, V.; Galinier, A.; Boel, G.; Maze, A.; Deutscher, J.
HPr kinase/phosphorylase, a Walker motif A-containing bifunctional sensor enzyme controlling catabolite repression in Gram-positive bacteria // Biochimica et Biophysica Acta - Proteins and Proteomics, 1697 (2004), 1-2; 123-135 (međunarodna recenzija, članak, znanstveni)
Poncet, S., Mijakovic, I., Nessler, S., Gueguen-Chaignon, V., Chaptal, V., Galinier, A., Boel, G., Maze, A. & Deutscher, J. (2004) HPr kinase/phosphorylase, a Walker motif A-containing bifunctional sensor enzyme controlling catabolite repression in Gram-positive bacteria. Biochimica et Biophysica Acta - Proteins and Proteomics, 1697 (1-2), 123-135.
@article{article, year = {2004}, pages = {123-135}, keywords = {protein kinase, P-protein phosphorylase, catabolite repression, inducer exclulsion, Gram-positive bacteria}, journal = {Biochimica et Biophysica Acta - Proteins and Proteomics}, volume = {1697}, number = {1-2}, issn = {1570-9639}, title = {HPr kinase/phosphorylase, a Walker motif A-containing bifunctional sensor enzyme controlling catabolite repression in Gram-positive bacteria}, keyword = {protein kinase, P-protein phosphorylase, catabolite repression, inducer exclulsion, Gram-positive bacteria} }
@article{article, year = {2004}, pages = {123-135}, keywords = {protein kinase, P-protein phosphorylase, catabolite repression, inducer exclulsion, Gram-positive bacteria}, journal = {Biochimica et Biophysica Acta - Proteins and Proteomics}, volume = {1697}, number = {1-2}, issn = {1570-9639}, title = {HPr kinase/phosphorylase, a Walker motif A-containing bifunctional sensor enzyme controlling catabolite repression in Gram-positive bacteria}, keyword = {protein kinase, P-protein phosphorylase, catabolite repression, inducer exclulsion, Gram-positive bacteria} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus





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