Plant Defence Signaling Network Inference Using Knowledge - Based Perturbations and Global Gene Expression Analysis (CROSBI ID 496449)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Genoud, Thierry ; Kulišić, Tea ; Trevino Santa Cruz, Marcela ; Metraux, Jean Pierre ; Pufky, Jean
engleski
Plant Defence Signaling Network Inference Using Knowledge - Based Perturbations and Global Gene Expression Analysis
In Arabidopsis three main signaling compounds activate the plant responses to pathogen attack: salicylic acid (SA), ethylene (ET), and jasmonic acid (JA). These substances and their different combinations can modulate the activation of groups of particular and/or similar genes. In order to determine the complexity of stimuli interpretation and to reconstitute the structure of the defense signaling network in Arabidopsis, we performed global analysis of gene expression (using microarray technology) of plants submitted to exogenous treatment with SA, ET, and JA, as well as their combinations (SA+JA, SA+ET, JA+ET, SA+JA+ET). Our concept of clustering is founded on preestablished signaling/genetic knowledge coupled with a set of highly significant perturbations allowing us to group genes in regulons with a high biological connectivity. The resulting classes determine the circuits of interpretation/combinations of cell defense signals. They have been inferred as minimal logical circuits, and represented in a Boolean formalism. In order to produce a representation of these statistical circuits inside a single graphical model, and to perform qualitative digital simulation, we developed a method for bio-digital circuits concatenation. As a first interesting result, signaling network analysis suggests that virulent pathogens block part of the defense signaling network in a semi specific manner.
plant defence signalling network
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Podaci o prilogu
54-54-x.
2003.
objavljeno
Podaci o matičnoj publikaciji
Proceedings of the International Conference "Pathways, Networks, and Systems: Theory and Experiments"
Aegean Conferences Series
Santorini: The Nomikos Center
Podaci o skupu
Pathways, Networks and Systems: Theory and Experiment
poster
29.09.2003-02.10.2003
Santorini, Grčka