engleski

Yeast non-replicative plasmids and strain construction

Stable transformation with yeast non-replicative vectors relies on homologous recombination between yeast chromosome and plasmid molecule. We show that UV-irradiation of double-stranded plasmids stimulates this process. Irradiation of either double or single-stranded non-replicative plasmids does not lead to detectable mutagenesis. Transformation by conversion of point mutations is favored if single-stranded plasmids are UV-irradiated. Linearized single-stranded plasmids may even convert the insertions of the Ty element (6.1 kb).

yeast; transformation; non-replicative plasmids; gene conversion

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