Yeast non-replicative plasmids and strain construction (CROSBI ID 496380)
Prilog sa skupa u zborniku | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Zgaga, Zoran ; Koren, Predrag ; Ninković, Milena ; Gjuračić, Krešimir ; Alačević, Marija
engleski
Yeast non-replicative plasmids and strain construction
Stable transformation with yeast non-replicative vectors relies on homologous recombination between yeast chromosome and plasmid molecule. We show that UV-irradiation of double-stranded plasmids stimulates this process. Irradiation of either double or single-stranded non-replicative plasmids does not lead to detectable mutagenesis. Transformation by conversion of point mutations is favored if single-stranded plasmids are UV-irradiated. Linearized single-stranded plasmids may even convert the insertions of the Ty element (6.1 kb).
yeast; transformation; non-replicative plasmids; gene conversion
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Podaci o prilogu
327-330-x.
1994.
objavljeno
Podaci o matičnoj publikaciji
Alberghina, L ; Frontali, L ; Sensi, P
New York (NY): Elsevier
Podaci o skupu
Nepoznat skup
poster
29.02.1904-29.02.2096