Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 147365

Yeast transformation with non-replicative plasmids: different genetic alterations and the choice of the vector


Gjuračić, Krešimir; Ninković, Milena; Koren, Predrag; Alačević, Marija; Zgaga, Zoran
Yeast transformation with non-replicative plasmids: different genetic alterations and the choice of the vector // Proceedings of the 2nd Croatian Congress of Food Technologists, Biotechnologists and Nutritionists
Zagreb, Croatia: Prehrambeno-tehnološka i biotehnološka revija 32 (4), 1994. str. 163-166 (poster, domaća recenzija, cjeloviti rad (in extenso), znanstveni)


CROSBI ID: 147365 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Yeast transformation with non-replicative plasmids: different genetic alterations and the choice of the vector

Autori
Gjuračić, Krešimir ; Ninković, Milena ; Koren, Predrag ; Alačević, Marija ; Zgaga, Zoran

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Proceedings of the 2nd Croatian Congress of Food Technologists, Biotechnologists and Nutritionists / - Zagreb, Croatia : Prehrambeno-tehnološka i biotehnološka revija 32 (4), 1994, 163-166

Skup
2nd Croatian Congress of Food Technologists, Biotechnologists and Nutritionists

Mjesto i datum
Zagreb, Hrvatska, 15-17.06.1994

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
yeast; transformation; homologous recombination; illegitimate recombination; UV irradiation

Sažetak
The efficiency of yeast transformation with non-replicative plasmids, as well as the type of genetic alteration introduced, can be significantly influenced by different in vitro modifications of the plasmid. The effects of conformation of the plasmid molecule (double- or single-stranded form), plasmid linearization with restriction enzymes, DNA divergence between chromosomal and plasmidic sequences and UV-irradiation of the plasmid have been studied. These investigations can help us to better understand the mechanisms of genetic recombination but they might also be useful for improving the techniques of yeast strain construction. Introduction of foreign DNA fragments into the yeast genome by illegitimate integration of single-stranded plasmids or increasing of transformation efficiency by UV-irradiation of the plasmid are some of the possible applications of these investigations.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA



Citiraj ovu publikaciju

Gjuračić, Krešimir; Ninković, Milena; Koren, Predrag; Alačević, Marija; Zgaga, Zoran
Yeast transformation with non-replicative plasmids: different genetic alterations and the choice of the vector // Proceedings of the 2nd Croatian Congress of Food Technologists, Biotechnologists and Nutritionists
Zagreb, Croatia: Prehrambeno-tehnološka i biotehnološka revija 32 (4), 1994. str. 163-166 (poster, domaća recenzija, cjeloviti rad (in extenso), znanstveni)
Gjuračić, K., Ninković, M., Koren, P., Alačević, M. & Zgaga, Z. (1994) Yeast transformation with non-replicative plasmids: different genetic alterations and the choice of the vector. U: Proceedings of the 2nd Croatian Congress of Food Technologists, Biotechnologists and Nutritionists.
@article{article, year = {1994}, pages = {163-166}, keywords = {yeast, transformation, homologous recombination, illegitimate recombination, UV irradiation}, title = {Yeast transformation with non-replicative plasmids: different genetic alterations and the choice of the vector}, keyword = {yeast, transformation, homologous recombination, illegitimate recombination, UV irradiation}, publisher = {Prehrambeno-tehnolo\v{s}ka i biotehnolo\v{s}ka revija 32 (4)}, publisherplace = {Zagreb, Hrvatska} }
@article{article, year = {1994}, pages = {163-166}, keywords = {yeast, transformation, homologous recombination, illegitimate recombination, UV irradiation}, title = {Yeast transformation with non-replicative plasmids: different genetic alterations and the choice of the vector}, keyword = {yeast, transformation, homologous recombination, illegitimate recombination, UV irradiation}, publisher = {Prehrambeno-tehnolo\v{s}ka i biotehnolo\v{s}ka revija 32 (4)}, publisherplace = {Zagreb, Hrvatska} }




Contrast
Increase Font
Decrease Font
Dyslexic Font