Subcellular localization of A and B Nm23/NDPK subunits (CROSBI ID 105179)
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Herak Bosnar, Maja ; de Gunzburg, Jean ; Bago, Ružica ; Brečević, Lukrecija ; Weber, Igor ; Pavelić, Jasminka
engleski
Subcellular localization of A and B Nm23/NDPK subunits
The human Nm23-H1/NDPK A and Nm23-H2/NDPK B encode for two subunits of the nucleoside diphosphate kinase - a ubiquitous enzyme that transfers the terminal phosphates from ATP to (d)NDPs. Although having an 88% amino acid sequence identity and an, already, assigned role in the cell, the two subunits appear to have additional and distinctive cell functions. Both subunits, however, especially Nm23-H1 have been reported to be involved in tumor progression and metastasis. The aim of this study was to reveal the possible specific and, potentially, distinct localizations of the subunits in cells of different origin and differentiation stage using the GFP reporter system, and therefore, link the localization to the stage of disease. We analyzed the ectopic expression of GFP-Nm23 proteins in head and neck tumor cell lines by fluorescent microscopy techniques. Our experiments revealed that GFP-fused Nm23 proteins display identical and simultaneous localization being found in the cytosol and in the portion of the nuclei of all transfected cells regardless of their origin and differentiation status. Fluorescent immunocytochemical analysis revealed that the subunits share the localization with the endoplasmic reticulum.
nm23; head and neck tumor cell lines; endoplasmic reticulum; green fluorescent protein
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Povezanost rada
Temeljne medicinske znanosti, Biologija