engleski

Comparison of the API 20E and BBL Crystal E/NF identification systems for differentiating bacterial isolates from apparently healthy reared sea bass (Dicentrarcus labrax)

Two commercial rapid identification systems, API 20E and BBL Crystal E/NF, were compared as their ability to reliably identify bacterial isolates from internal organs of reared sea bass. The tests gave different results, API 20E identifying bacteria as Pseudomonas spp. with 37% accuracy, while BBL Crystal E/NF identified them as Flavobacterium odoratum with 99% accuracy. Although F. odoratum is not a marine fish pathogen, conventional tests conducted with the same isolates were more indicative of them being Flavobacterium spp. than Pseudomonas spp., suggesting that BBL Crystal E/NF was more reliable in this identification. Both systems were found to be applicable for diagnostics of marine fish pathogens, but should be used with caution because of possible misinterpretation.

API 20E ; BBL E/NF rapid tests ; sea bass

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