engleski

Interleukin-6 and its receptors in the CSF and serum of relapsing-remitting multiple sclerosis patients

Introduction: Inteleukin-6 (IL-6) is pleiotropic cytokine that influence antigen specific immune responses and inflammatory reactions. The functional receptor for IL-6 is a complex of signal transducing subunit glycoprotein 130 (sgp130) and the ligand binding subunit gp80 (sIL-6R). They interact to mediate intracellular signalling. Expression of IL-6 in multiple sclerosis (MS) is controversial and no convincing correlations have yet been made between IL-6 levels and disease activity The aim of this study was to measure IL-6 and its receptors in the cerebrospinal fluid (CSF) and serum from relapsing-remitting MS (RRMS) patients during acute exacerbation of the disease. Patients and methods: We analysed cerebrospinal fluid (CSF) and serum samples of 36 relapsing-remitting MS (RRMS) patients diagnosed according to the McDonald criteria during acute exacerbation of the disease, and 41 patients with noninflammatory neurological diseases (NIND), as controls. IL-6, sIL-6R and sgp130 levels were measured using commercial enzymeimmuno assay (ELISA). Student-t test was applied for statistical analysis of the data. Results: The results were expressed as mean &plusmn ; S.D. in pg/mL. Our results showed no statistical difference between all of the tested groups (p>0.05) and were as follows. Serum IL-6 level in RRMS was 18.06 &plusmn ; 47.58 and in NIND was 38.00 &plusmn ; 79.65. CSF IL-6 level in RRMS was 2.74 &plusmn ; 2.41 and in NIND group it was 2.26 &plusmn ; 2.37. Serum sIL-6R level was in RRMS 24845.33 &plusmn ; 11117.87 and in NIND was 22739.16 &plusmn ; 10098.05. CSF level of sIL-6R was in RRMS 528.99 &plusmn ; 467.95 and in NIND it was 484.51 &plusmn ; 789.43. Serum sgp130 levels were in RRMS group 710950 &plusmn ; 82950 and in NIND 801970 &plusmn ; 128911. The CSF sgp130 level was in RRMS 30470 &plusmn ; 29750 and in NIND was 38450 &plusmn ; 27210. Conclusion: Our results suggest that IL-6 and its soluble receptors (sIL-6R and sgp130) are probably not altered in RRMS during acute exacerbation of the disease.

Il-6; gp130; sIL-6R; multiple sclerosis

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