engleski

Detrmination of Glucosa-oxydase Kinetic Constants Using Potentiometric Method

The oxidation reaction of D(+)glucose, catalyzed by the enzyme glucose-oxydase, as well as the quantity of produced gluconic acid, have been followed by direct potentiometry and potentiometric titration method. The optimal conditions for the enzyme action have been established (enzyme and substrate concentration, pH, temperature, enzyme stability in water solution, inhibitors). The kinetic constatnts of the investigated enzyme have also been determined.

glucose-oxsydase; kinetic constatns; potentiometry

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