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Pregled bibliografske jedinice broj: 139628

Determination of oxalate in urine, using an amperometric biosensor with oxalate oxidase immobilized on the surface of a chromium hexacyanoferrate-modified graphite electrode


Milardović, Stjepan; Grabarić, Zorana; Tkalčec, Mihael; Rumenjak, Vlatko
Determination of oxalate in urine, using an amperometric biosensor with oxalate oxidase immobilized on the surface of a chromium hexacyanoferrate-modified graphite electrode // Journal of AOAC International, 83 (2000), 1212-1217 (međunarodna recenzija, članak, znanstveni)


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Naslov
Determination of oxalate in urine, using an amperometric biosensor with oxalate oxidase immobilized on the surface of a chromium hexacyanoferrate-modified graphite electrode

Autori
Milardović, Stjepan ; Grabarić, Zorana ; Tkalčec, Mihael ; Rumenjak, Vlatko

Izvornik
Journal of AOAC International (1060-3271) 83 (2000); 1212-1217

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Flow-injection analysis. Enzyme electrode. Hydrogen-peroxide. Ferrocene. Fructose. Samples. Sensor. Assay

Sažetak
A novel enzymatic amperometric method is described for the determination of oxalic acid in urine. An amperometric biosensor was made by immobilizing oxalate oxidase on the surface of a chromium(III) hexacyanoferrate-modified graphite electrode by using a bovine serum albumin and glutaraldehyde cross-linking procedure. The enzyme biocatalyzes oxalate decomposition in the presence of oxygen into carbon dioxide and hydrogen peroxide, The oxalate concentration, which is proportional to the amount of hydrogen peroxide, was determined amperometricaily by measuring the current resulting in the reduction of hydrogen peroxide at a very low working potential (0.05 V versus the Hg \ Hg2Cl2 \ 3M KCl electrode), which minimized the influence of the possible interferences present in human urine. All experiments were performed with succinic buffer, pH 3.8, containing 0.1 M KCl and 5.4 mM ethylenediaminetetraacetic acid. In an aqueous solution of pure oxalic acid, the biosensor showed good linearity in a concentration range of 2.5-100 mu M without the use of a dialysis membrane. For untreated urine samples, a high correlation (R-2 = 0.9949) was Obtained between oxalate concentrations added to urine samples and oxalate recoveries calculated for determinations with the described oxalate biosensor.

Izvorni jezik
Engleski



POVEZANOST RADA



Citiraj ovu publikaciju

Milardović, Stjepan; Grabarić, Zorana; Tkalčec, Mihael; Rumenjak, Vlatko
Determination of oxalate in urine, using an amperometric biosensor with oxalate oxidase immobilized on the surface of a chromium hexacyanoferrate-modified graphite electrode // Journal of AOAC International, 83 (2000), 1212-1217 (međunarodna recenzija, članak, znanstveni)
Milardović, S., Grabarić, Z., Tkalčec, M. & Rumenjak, V. (2000) Determination of oxalate in urine, using an amperometric biosensor with oxalate oxidase immobilized on the surface of a chromium hexacyanoferrate-modified graphite electrode. Journal of AOAC International, 83, 1212-1217.
@article{article, year = {2000}, pages = {1212-1217}, keywords = {Flow-injection analysis. Enzyme electrode. Hydrogen-peroxide. Ferrocene. Fructose. Samples. Sensor. Assay}, journal = {Journal of AOAC International}, volume = {83}, issn = {1060-3271}, title = {Determination of oxalate in urine, using an amperometric biosensor with oxalate oxidase immobilized on the surface of a chromium hexacyanoferrate-modified graphite electrode}, keyword = {Flow-injection analysis. Enzyme electrode. Hydrogen-peroxide. Ferrocene. Fructose. Samples. Sensor. Assay} }
@article{article, year = {2000}, pages = {1212-1217}, keywords = {Flow-injection analysis. Enzyme electrode. Hydrogen-peroxide. Ferrocene. Fructose. Samples. Sensor. Assay}, journal = {Journal of AOAC International}, volume = {83}, issn = {1060-3271}, title = {Determination of oxalate in urine, using an amperometric biosensor with oxalate oxidase immobilized on the surface of a chromium hexacyanoferrate-modified graphite electrode}, keyword = {Flow-injection analysis. Enzyme electrode. Hydrogen-peroxide. Ferrocene. Fructose. Samples. Sensor. Assay} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • Chemical Abstracts





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