engleski

Analysis of phenolic components in Croatian red wines from different geographical origins by TLC

Thin-layer chromatography (TLC) method are studied for determination of phenolic compounds in two samples of commercially available wines from the Croatia. Sample preparation method using liquid-liquid extraction with diethyl ether at pH 2.0 was used. Extracts and standard solutions were applied on silica gel 60 F254 TLC plates (20 × 20 cm). The efficiency of eleven mobile phases, used for this purpose, has been tested by three mathematical techniques: calculation of the information content (I) derived from Shannon 's equation ; determination of discriminating power (DP) and formation of clusters and dendrogram. It has been shown that the most favourable mobile phase for separation phenolic compounds in extract of wine “ Frankovka” is benzene-ethyl acetate-formic acid, 30 + 15 + 5 (v/v), while for wine "Merlot" optimal mobile phases are chloroform-methanol-formic acid, 15 + 3 + 2 (v/v) and ethyl acetate-chloroform-formic acid, 24 + 21 + 5 (v/v). Six phenolic compounds have been identified in the tested samples.

flavonoids ; red wines ; TLC ; numerical methods

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