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Pregled bibliografske jedinice broj: 137325

Application of semi-industrial monolithic columns for downstream processing of clotting factor IX.


Branović, Karmen; Buchacher, A.; Barut, Miloš; Štrancar, Aleš; Josić, Đuro
Application of semi-industrial monolithic columns for downstream processing of clotting factor IX. // Journal of Chromatography B-Analitical Technologies in the Biomedical and Life Sciences, 790 (2003), 1-2; 175-182 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 137325 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Application of semi-industrial monolithic columns for downstream processing of clotting factor IX.

Autori
Branović, Karmen ; Buchacher, A. ; Barut, Miloš ; Štrancar, Aleš ; Josić, Đuro

Izvornik
Journal of Chromatography B-Analitical Technologies in the Biomedical and Life Sciences (1570-0232) 790 (2003), 1-2; 175-182

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
factor IX; purification; DEAE-Sephadex; monolithic columns; human
(faktor IX; pročišćavanje; DEAE-Sephadex; monolitne kolone; čovjek)

Sažetak
It has been shown in a previous study that monolithic columns can be used for downstream processing of different concentrates of clotting factor IX [K. Branovic et al., J. Chromatogr. A 903 (2000) 21]. This paper demonstrates that such supports are useful tools also at an early stage of the purification process of factor IX from human plasma. Starting with the eluate after solid-phase extraction with DEAE-Sephadex, the use of monolithic columns has allowed much better purification than that achieved with conventional anion-exchange supports. The period of time required for separation is also much reduced. In up-scaling experiments, separations are carried out with 8, 80 and 500 ml columns. A volume of 1830 ml of DEAE-Sephadex eluate, containing a total of 27.6 g of protein and 48500 IU of factor IX is applied to the 500 ml monolithic column. This corresponds to a separation on a pilot scale. The results of this separation after up-scaling are comparable to those obtained with the 8 ml column on a laboratory scale.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita



POVEZANOST RADA


Projekti:
0021001

Ustanove:
Imunološki zavod d.d.

Profili:

Avatar Url Karmen Branović-Čakanić (autor)


Citiraj ovu publikaciju:

Branović, Karmen; Buchacher, A.; Barut, Miloš; Štrancar, Aleš; Josić, Đuro
Application of semi-industrial monolithic columns for downstream processing of clotting factor IX. // Journal of Chromatography B-Analitical Technologies in the Biomedical and Life Sciences, 790 (2003), 1-2; 175-182 (međunarodna recenzija, članak, znanstveni)
Branović, K., Buchacher, A., Barut, M., Štrancar, A. & Josić, Đ. (2003) Application of semi-industrial monolithic columns for downstream processing of clotting factor IX.. Journal of Chromatography B-Analitical Technologies in the Biomedical and Life Sciences, 790 (1-2), 175-182.
@article{article, year = {2003}, pages = {175-182}, keywords = {factor IX, purification, DEAE-Sephadex, monolithic columns, human}, journal = {Journal of Chromatography B-Analitical Technologies in the Biomedical and Life Sciences}, volume = {790}, number = {1-2}, issn = {1570-0232}, title = {Application of semi-industrial monolithic columns for downstream processing of clotting factor IX.}, keyword = {factor IX, purification, DEAE-Sephadex, monolithic columns, human} }
@article{article, year = {2003}, pages = {175-182}, keywords = {faktor IX, pro\v{c}i\v{s}\'{c}avanje, DEAE-Sephadex, monolitne kolone, \v{c}ovjek}, journal = {Journal of Chromatography B-Analitical Technologies in the Biomedical and Life Sciences}, volume = {790}, number = {1-2}, issn = {1570-0232}, title = {Application of semi-industrial monolithic columns for downstream processing of clotting factor IX.}, keyword = {faktor IX, pro\v{c}i\v{s}\'{c}avanje, DEAE-Sephadex, monolitne kolone, \v{c}ovjek} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE





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