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Pregled bibliografske jedinice broj: 136117

Mutational analysis of basic residues in the N-terminal part of the rRNA : m6A methyltransferase ErmC'


Maravić, Gordana; Bujnicki, Janusz M.; Flögel, Mirna
Mutational analysis of basic residues in the N-terminal part of the rRNA : m6A methyltransferase ErmC' // Folia Microbiologica, 49 (2004), 1; 3-7 (međunarodna recenzija, članak, znanstveni)


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Naslov
Mutational analysis of basic residues in the N-terminal part of the rRNA : m6A methyltransferase ErmC'

Autori
Maravić, Gordana ; Bujnicki, Janusz M. ; Flögel, Mirna

Izvornik
Folia Microbiologica (0015-5632) 49 (2004), 1; 3-7

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
site-directed mutagenesis; antibiotic resistance; methylation; RNA binding; catalysis

Sažetak
Erm methyltransferases mediate the resistance to the macrolide-lincosamide-streptogramin B antibiotics via dimethylation of a specific adenine residue in 23 S rRNA. In spite of the biomedical importance of this process, virtually nothing is known about the function of N- and C-terminal sequence regions, which are unique to Erm methyltransferases. To define the role of positively charged N-terminal residues of the ErmC' methyltransferase in RNA binding and/or catalysis, we have undertaken the mutational analysis of amino acids K4 and K7 and characterized mutants in vivo and in vitro. Our results suggest that K4 and K7 residues are dispensable for the enzyme activity, however they seem to considerably support the catalytic step of the reaction, probably by maintaining the optimal conformation of the transition state through interactions with the phosphate backbone of RNA.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
0006611

Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb

Citiraj ovu publikaciju

Maravić, Gordana; Bujnicki, Janusz M.; Flögel, Mirna
Mutational analysis of basic residues in the N-terminal part of the rRNA : m6A methyltransferase ErmC' // Folia Microbiologica, 49 (2004), 1; 3-7 (međunarodna recenzija, članak, znanstveni)
Maravić, G., Bujnicki, J. & Flögel, M. (2004) Mutational analysis of basic residues in the N-terminal part of the rRNA : m6A methyltransferase ErmC'. Folia Microbiologica, 49 (1), 3-7.
@article{article, year = {2004}, pages = {3-7}, keywords = {site-directed mutagenesis, antibiotic resistance, methylation, RNA binding, catalysis}, journal = {Folia Microbiologica}, volume = {49}, number = {1}, issn = {0015-5632}, title = {Mutational analysis of basic residues in the N-terminal part of the rRNA : m6A methyltransferase ErmC'}, keyword = {site-directed mutagenesis, antibiotic resistance, methylation, RNA binding, catalysis} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE





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