engleski

Polymorphisms of CETP gene in patients with coronary artery disease (Zagreb cohort)

Cholesteryl ester transfer protein (CETP) has a central role in the metabolism of HDL and might alter the susceptibility to atherosclerosis. The aim of our study was to determine whether C(-628)A, I405V, or D442G variants of the CETP gene have an impact on the risk of developing coronary artery disease (CAD). Based on angiography results, the patients were divided into CAD (n=479) and control (n=200) groups. Genotyping was performed by multi-target PCR followed by detection of amplified alleles with linear arrays of immobilized sequence-specific probes on nylon membrane strips. All the genotypes were in Hardy-Weinberg equilibrium. Odds ratios (OR) for the genotypes were calculated by logistic regression after adjusting for age, sex, log (triglyceride), total and HDL-cholesterol, lipoprotein (a), fibrinogen, diabetes, hypertension and smoking. The VV genotype of the CETP gene was associated with increased risk of developing CAD (OR 2.32, 95% CI 1.07-5.04, p=0.035). CETP C(-628)A variant was not associated with CAD. Only one patient with D442G variant was found in the CAD group. Multivariate analysis did not show significant effect of I405V variant on serum concentrations of lipids. However, AA genotype in the promotor was associated with higher concentrations of HDL-cholesterol, its subfractions and apolipoprotein AI (p < 0.001). We conclude that the VV genotype bearers are at significant risk of developing CAD in Croatian population and AA genotype is a determinant of HDL-cholesterol and apolipoprotein AI levels.

CETP gene; coronary artery disease; HDL-cholesterol

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