engleski

Changes in DNA methylation during somatic embryogenesis in Cucurbita pepo L.

Three pumpkin embryogenic lines were initiated on wounded zygotic embryos cultured on medium with or without 2, 4-dichlorophenoxyacetic acid (2, 4-D). Somatic embryo development was controlled by the availability of various compounds in the medium: presence/absence of 2, 4-D, nitrogen sources. The highest rate of DNA methylation was in the early embryo stages, predominantly on MSC medium with 2, 4-D and on auxin-free medium supplemented with 1.0 mM NH4Cl. DNA methylation was correlated with early embryo development in a manner that was not exclusively dependent on the presence/absence of exogenous auxin. DNA methylation decreased during embryo maturation on auxin-free MSC medium and on auxin-free MSC supplemented with 12.3 uM 5- azacytidine (5-azaC). The embryogenic features of the pumpkin tissue were preserved, even after a 2- month treatment with 5-azaC.

5-azacytidine ; Cucurbita pepo L. ; Hormone-free medium ; DNA methylation ; Somatic embryogenesis

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