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izvor podataka: crosbi

Coexistence of Escherichia coli and Salmonella enterica serovar Typhimurium during prolonged starvation in stationary phase (CROSBI ID 103390)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Bačun-Družina, Višnja ; Čulić, Valentina ; Rosandić, Lucija ; Franekić Čolić, Jasna Coexistence of Escherichia coli and Salmonella enterica serovar Typhimurium during prolonged starvation in stationary phase // Periodicum biologorum, 104 (2002), 4; 425-430

Podaci o odgovornosti

Bačun-Družina, Višnja ; Čulić, Valentina ; Rosandić, Lucija ; Franekić Čolić, Jasna

engleski

Coexistence of Escherichia coli and Salmonella enterica serovar Typhimurium during prolonged starvation in stationary phase

The natural selection could occur in cultures of Enterobacteriaceae mantained during prolonged stationary phase. The resulting mutants with increased fitness express GASP enabling them to grow and displace the parent as the majority population. The purpose of our investigation was to analyse mixed populations of Escherichia coli K12 and Salmonella enterica serovar Typhimurium, during the prolonged period of carbon starvation. The homogeneous or mixed cultures were incubated with aeration at 37°C in glass test tubes. We determined viable cell counts by making serial dilutions in PBS buffer and plating onto LB, and selective minimal lactose or Simmons citrate plates. The results showed that after ten days of growth of a single and mixed bacterial species in complete medium the growth rate considerably decreased compared to the growth in minimal medium. In the mixed cultures during eleven days of growth in minimal medium, the aged cells or the rpoS mutant of E. coli, do not have ability to overgrow the younger or the cells of the same age of another bacterial species. The mixture of one-day-old cultures of S. enterica and sixteen days old cells of E. coli as minority did not show the GASP phenomenon. The same results were obtained if the mixture consisted of the S. enterica as minority cells.

Escherichia coli; Salmonella enterica serovar Typhimurium; growth advantage in stationary phase (GASP); carbon starvation stress; rpoS gene.

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

104 (4)

2002.

425-430

objavljeno

0031-5362

Povezanost rada

Biotehnologija

Indeksiranost