engleski

Differentiation and developmental potential of rat postimplantation embryo without extraembryonic membranes cultured in vitro or grafted in vivo

Different experimental systems are used to study developmental processes in mammals. In this study three experimental models were analyzed and correlated: 1) cultivation of rat embryos in vitro ; 2) cultivation in vitro and then transplantation in vivo ; 3) direct transplantation in vivo. When embryos were cultivated in vitro and then transplanted in vivo, after the initial in vitro restriction developmental potential was recovered. The in vitro restriction depended on medium used and duration of culture. Precultivation in serum-free medium for 7 days restricted developmental potential for nervous tissue, and for 14 days restricted developmental potential for skeletal muscles, adipose tissue and glandular epithelia. Transferrin addition improved in vitro differentiation of neuroblasts, carilage and columnar epithelium. In combined in vitro and in vivo method transferrin preserved developmental potential in comparable extent as the addition of the serum. Even in serum-free conditions in vitro, the subsequent in vivo wide expression of developmental potential was possible. Therefore the combination of in vitro and in vivo methods turned to be advantageous to the isolated approaches (in vitro or in vivo only), and enabled testing in more detail the influence of a single substance on developmental course and potential.

embryo culture; embryo grafts; serum-free; transferrin

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