engleski

Investigation of the antimutagenic effect of Cruciferae vegetables on bacterial test systems and human cell lines

Glucosinolates are group of compounds widely distributed among the plants of Cruciferae family. These compounds are easily hydrolysed by the enzyme myrosinase which becomes activated after mechanical drstruction of the plant cells. Isothiocyanates, nitriles, thiocyanates and indole compounds are the most common products of such hydrolysis. In our previous report we have shown that there was no cytotoxic and mutagenic effect of indole-3-carbinol and cauliflower extract on wo bacterial test systems, Salmonella typhimurium, strains TA98 and TA100. In this work we have examined potential inhibitory effect of both compounds against two standard mutagens, 2-aminoantrachene and 4-nitroquinoline-1-oxide. It was estimated that simultaneous treatment of bacterial cells with mutagen and indole compound leads to a significiant inhbition of prototrofic growth. Indole compounds didn't inhibit the activity of P-glycoprotein in parental laryngeal carcinoma cells (HEp") and their cisplatin resistant subline (CK2). Indole compounds caused significant increase of glutathione level and glutathione-S-transferase activity in HEp2 cells . On the contrary, in CK2 cells which have uncreased basal level of glutathione, both drugs decreased the level of glutathione and glutathione-S-transferase activity. Indole-3-carbinol and cauliflower extract didn't cause any aberrant event in human lympocytes.

Cruiferae family; Salmonella typhimurium; Indole-3-carbinol

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