engleski

Alkaline phosphatase isoenzymes in benign transitory hyperphosphatasemia

Introduction:Transitory hyperphosphatasemia is a rare disorder of marked increase in total alkaline phosphatase (AP) activity. It usually occurs in childhood, has no specific symptoms, and is detected by accident. Patients and methods: From April 2001 till November 2002, seven children (mean age 12.9 months, range 7-21 months) with extremely high serum AP activity were seen. An associated increase in the 5-nucleotidase activity was detected in five children. They were hospitalized for various reasons like respiratory infection, enteropathy, anemia and preoperative screening. Based on laboratory findings, radiologic and clinical examination, hepatic disease and metabolic bone disease were excluded. AP was determined by the IFCC method using Olympus reagents on an AU 600 Olympus autoanalyzer. AP isoenzymes were determined on Beckman Isopal Plus agarose gel. Temperature denaturation at 56 &#61616 ; ; C/10 min and neuroaminidase treatment were used for better separation of the bone and hepatic isoenzymes. Results: The pooled mean AP activity in the study children was 3665 IU/L (range 1628-5469 IU/L). Isoenzyme separation demonstrated the bone isoenzyme to predominantly contribute to the total AP activity increase. Five children also showed a mild hepatic isoenzyme elevation. The AP activity normalized spontaneously in a mean of 5.1 months (range 1.5-9 months). Conclusion:Timely recognition of this biochemical phenomenon can help avoid the traps of false diagnosis and unnecessary testing.

transitory hyperphosphatasemia; alkaline phosphatase; children

nije evidentirano