engleski

Nitric oxide production by peritoneal macrophages induced by pentapeptide-dissaccharide from B. divaricatum and some of its derivatives

Low molecular weight bacterial peptidoglycans like muramyl peptides are immunomodulators usually acting indirectly through activation of macrophages. They are generally less toxic but in the same time less potent than large bacterial cell wall fragments. Therefore, many derivatives of muramyl peptides have been constructed with the aim to inprove their activity. Here we present the data of the influence of one dissaccharide-pentapeptide from Brevibacterium divaricatum (PGM), its lypophilic (Na-Lauroil PGM) and metalo derivative (Fe-PGM) as well as two adamantyl-dipeptide stereoisomers (AdTP1 and AdTP2) on NO production by mouse peritoneal macrophages. PGM and its Na-lauroil derivative alone increased the production of NO by peritoneal macrophages, however less than LPS did. Fe-PGM and adamantyl dipeptides alone were ineffective. In combination with IFN both adamantyl dipeptides , but not PGM , expressed synergistic effect on the NO production (up to the level of LPS stimulatory effects.) In combination with LPS only one stereoisomere of adamantyl-dipeptide (AdTP2) showed synergistic stimulatory effect.

nitric oxide; peptidoglycans; immunomodulation

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