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Pregled bibliografske jedinice broj: 128550

Proportion of lymphoid and myeloid cell subsets in mesenteric lymph node of pigs immunized with F4ac^+ nonenetrotoxigenic Escherichia coli strain


Šver, Lidija; Haverson, Karin; Oršolić, Nada; Terzić, Svjetlana; Tomljenović, Andrea; Valpotić, Ivica
Proportion of lymphoid and myeloid cell subsets in mesenteric lymph node of pigs immunized with F4ac^+ nonenetrotoxigenic Escherichia coli strain // Annual Meeting of the Croatian Immunological Society 2002.
Trakošćan, Hrvatska, 2002. str. 45-45 (poster, domaća recenzija, sažetak, znanstveni)


Naslov
Proportion of lymphoid and myeloid cell subsets in mesenteric lymph node of pigs immunized with F4ac^+ nonenetrotoxigenic Escherichia coli strain

Autori
Šver, Lidija ; Haverson, Karin ; Oršolić, Nada ; Terzić, Svjetlana ; Tomljenović, Andrea ; Valpotić, Ivica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Annual Meeting of the Croatian Immunological Society 2002. / - , 2002, 45-45

Skup
Annual Meeting of the Croatian Immunological Society 2002

Mjesto i datum
Trakošćan, Hrvatska, 22-24.11.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Escherichia coli; F4ac; intragastric immunization; mesenteric lymph node

Sažetak
To evaluate the immunogenicity of F4ac^+ non-enterotoxigenic Escherichia coli (non-ETEC) vaccine candidate strain in 4-week-old pigs we have used monoclonal antibodies (mAbs) reactive with swine leukocyte CD/SWC antigens to determine eventual changes in the immunophenotypic expression of these molecules and the proportion of CD+/SWC+ immune cells from mesenteric lymph node (MLN) by flow cytometry. Intragastric immunization of pigs with 10^10 CFU/ml of F4ac^+ non-ETEC strain Abbotstown A induced changes in the proportion of lymphoid and myeloid cells in MLN of immunized pigs as compared to those recorded in unimmunized pigs. The presence of antigen-specific T (CD3, CD4, CD8, CD25, CD45RC, CD45RA) and B cell (CD21, CD45RC, CD45RA) subsets and antigen presenting cells (APCs ; SWC3, CD14) demonstrates that the compartment of gut-associated lymphoid tissue of weaned pigs, i.e. MLN, is functionally organized for the expression of immune responses to the bacterial immunogens, such as F4 fimbriated non-ETEC tested as a potential source vaccinal antigen.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina



POVEZANOST RADA


Projekt / tema
0048003
0053305

Ustanove
Hrvatski veterinarski institut, Zagreb,
Veterinarski fakultet, Zagreb