engleski

Cytotoxicity of Three Root Canal Sealers

The objective of this study was to evaluate the cytotoxicity of three commercialy aviable root canal sealers: APEXIT, AH PLUS and KETAC-ENDO by using mouse L929 fibroblasts.Freshly mixed sealers, prepared according to the manufacturers' directions, were placed on teflon discs and put on the well bottom of a 12-well plate. A teflon disc, with no material added, served as control. The sealers were covered with cell suspension and incubated at 37&ordm ; C for 1, 6, 20 and 24 hours. After incubation, the teflon discs were removed, and the morphological changes and number of viable cells were determined microscopically. Ketac Endo caused a significant decrease in the number of viable L929 cells after 1 hour, while no viable cells erved after 6 hours. AH Plus reduced significantly the cell number for the 6 h, 20 h and 24 h incubation times. The effect of Apexit on viable cells was not statistically significant. The least cytotoxic sealer was Apexit followed by AH Plus and Ketac Endo.

Root canal sealers ; Biocompatibility ; Cytotoxicity

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