engleski

CHANGE IN OPIORPHIN LEVELS IN SALIVA AFTER CAPSAICIN STIMULATION - COMPARISON OF OROFACIAL PAIN PATIENTS AND THE CONTROLS

Aim: The aim of the study was to compare the intensity of burning pain sensation and salivary opiorphin levels after stimulation with capsaicin in burning mouth syndrome (BMS) patients, temporomandibular disorders (TMD) patients and healthy subjects. Opiorphin is an endogenous peptide isolated from saliva that has an exceptional analgesic effect, and its concentration is expected to be higher in subjects with chronic pain disorders. Materials and Methods: The study involved 48 subjects (16 BMS patients, 16 TMD patients and 16 controls). The burning pain sensation was induced by a capsaicin solution applied to a set of 10 discs during five minute time period. Subjects recorded the intensity of the stimulus using the Numerical Pain Rating Scale (NPRS) at precisely determined time points (every minute during 5 minutes of stimulation and next 20 minutes after the end of disc application). Saliva was collected three times, before capsaicin stimulation (1st sampling), immediately after the end of stimulation (2nd sampling) and 20 minutes after the end of stimulation (3rd sampling). Opiorphin levels were quantified by HPLC-MS / MS. Friedman ANOVA, Kruskal Wallis ANOVA and Mann Whitney U Test were used for statistical analysis. Results: From 9th minute until the end of the measurement, the values of the experimentally induced burning pain sensation were significantly higher in BMS group compared to healthy subjects and TMD patients (p<0.05). TMD patients showed statistically higher levels of opiorphin compared to control group in 1st (p=0.002) and 3rd sampling (p=0.022). BMS patients showed statistically higher levels of opiorphin compared to control group in 2nd (p=0.021) and 3rd sampling (p=0.004). In addition, a statistically significant increase in opiorphin levels was observed in BMS group between the 1st and 3rd sampling (p=0.011). In patients with orofacial pain conditions opiorphin levels were generally higher compared to healthy subjects. In addition, a more intense reaction to the experimental stimulus with capsaicin was observed in BMS group. Concusion: These results indicate a specific regulation of opiorphin release and possibly an altered response pattern to painful stimuli in patients with orofacial pain conditions.

Burning Mouth Syndrome (BMS) ; Temporomandibular Disorders (TMD ; Capsaicin ; Opiorphin

nije evidentirano