engleski

Association of catechol-o-methyltransferase and opiorphin gene polymorphisms with temporomandibular disorders

Aim: The aim of this study was to assess the association between single nucleotide polymorphisms (SNPs) in COMT (catechol-O- methyltransferase) and OPRPN (opiorphin) genes with temporomandibular disorders. Materials and Methods: The case-control study included 170 subjects: 85 patients diagnosed with pain-related temporomandibular disorders (TMDp) (according to Diagnostic Criteria for TMD) and 85 healthy controls (CTR). Characteristic Pain Intensity, assessed using the Graded Chronic Pain Scale, divided TMDp patients into high (HPI) and low intensity pain (LPI) groups. Genomic DNA was extracted from buccal mucosa swabs and used in the analysis of SNPs in COMT (rs4680, rs4818) and OPRPN (rs1387964) genes. SNP analysis was performed by qPCR using the TaqMan SNP Genotyping assays. Genotype distribution between studied groups was analyzed with respect to dominant and recessive genetic models. In both models, the minor allele represented the risk allele. The recessive model estimated the frequency of carriers of both minor alleles while the dominant model estimated the frequency of carriers of one or both minor alleles. Results: We didn’t find any significant difference in genotype frequencies of COMT rs4680 and rs4818 SNPs between TMDp and CTR groups. Two minor allele carriers (CC genotype) of rs1387964 polymorphism were significantly more frequent in TMDp than in CTR group (12.9% vs. 3.5%, p=0.025). When comparing HPI to LPI groups, we did not observe any significant difference in the genotype distribution of rs1387964 SNP. Two minor allele carriers (GG genotype) of rs4680 and rs4818 SNPs were significantly more represented in HPI compared to the LPI group (40% vs. 11.4% ; 24% vs. 7.5%, respectively). Conclusion: This study provides evidence that CC genotype of rs1387964 SNP in OPRPN gene might be associated with painful TMD, while GG genotypes of rs4680 and rs4818 SNPs in COMT gene appear to be associated with pain intensity. If confirmed in further research, determining the genetic predisposition for TMD could contribute to the development of a genetic test that could assess the risk of developing the disease

Temporomandibular Disorders ; Genotyping ; Pain ; Single Nucleotide Polymorphism

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