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Synergy of NMR and FTIR spectroscopy in the characterization of asymmetric liposomes

Although asymmetric liposomes are nowadays considered the most suitable representatives of plasma membranes, their rather challenging preparation accompanied by the use of limiting combinations of lipids, is further complicated by their far from straightforward characterization. [1] One of the indispensable techniques in confirming the asymmetric distribution of exclusively phosphatidylcholine (PC) lipids is 1H NMR spectroscopy, which, in the presence of lanthanide ions such as Pr3+, can distinguish the protons of choline group (−N(CH3)3+) located in the inner or outer membrane leaflet (Figure 1).[2] With the aim of identifying PC lipid molecules in a certain leaflet of asymmetric liposomes, we prepared them from 1, 2-dipalmitoyl-sn-glycero-3- phosphocholine (DPPC) and 1, 2-dipalmitoyl-sn- glycero-3-phosphoserine (DPPS), while the response of −N(CH3)3+ group was characterized with 1H NMR and for the first time by FTIR spectroscopy.

Asymmetric liposomes ; NMR spectroscopy ; DSC ; FTIR spectroscopy

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