Strict Regulation of the KEAP1-NRF2 Pathway and its Interaction with the Aquaporin 3 Expression in Breast Cancer Cell Lines (CROSBI ID 737325)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Mlinarić, Monika ; Čipak Gašparović, Ana
engleski
Strict Regulation of the KEAP1-NRF2 Pathway and its Interaction with the Aquaporin 3 Expression in Breast Cancer Cell Lines
Aquaporins (AQP) are membrane proteins that facilitate the transport of water and other small uncharged solutes across the plasma membrane, which makes them important in the regulation of cellular water homeostasis, proliferation, and migration. Several AQPs can transport hydrogen peroxide and are therefore named peroxiporins, who by facilitating hydrogen peroxide in and potentially out of the cell influence cell’s oxidative status. In the state of oxidative stress, characterized by an imbalance between oxidants and antioxidants, AQPs have a significant role and could contribute to oxidative damage in the cell. To prevent oxidative damage and associated undesirable effects, cells have a strong antioxidant system. As a major regulator of cellular resistance to oxidative stress, Nrf2 (nuclear factor erythroid 2–related factor 2) induces the expression of many antioxidant genes and therefore regulates cellular response to oxidative stress. Since it has an important role in fine-tuning of ROS, Nrf2 regulation is crucial. Keap1 (Kelch Like ECH Associated Protein 1) acts as a sensor for oxidative stress and is responsible for Nrf2 ubiquitination and degradation in physiological conditions, and its activation induced by oxidants. Once activated Nrf2 translocates to the nucleus where it exerts its effect. The Nrf2-Keap1 axis, as well as AQPs, are often dysregulated in tumors, who use them for their protection against constantly present oxidative stress. Our aim was to study the Nrf2 regulation in breast cancer cell lines, and to see does it somehow affect the AQP expression. According to the literature, AQP3 is often overexpressed in breast cancer cells compared to normal breast cells, so we put our efforts into finding its potential interaction with Nrf2. Our results indicate a strong regulation of Nrf2 activation independent of its expression. Cell line-dependent changes in AQP expression were also observed, but additional research is needed to determine whether there is an interaction between these proteins.
AQP3 ; NRF2 ; Keap1 ; breast cancer ; oxidative stress
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Podaci o prilogu
40-40.
2022.
nije evidentirano
objavljeno
978-953-48672-1-1
Podaci o matičnoj publikaciji
“HDIR-6: Targeting Cancer” The 6th Meeting of the Croatian Association for Cancer Research with International Participation : Book of Abstracts
Ozretić, Petar
Zagreb: Hrvatsko društvo za istraživanje raka (HDIR)
Podaci o skupu
“HDIR-6: Targeting Cancer” The 6th Meeting of the Croatian Association for Cancer Research with International Participation
poster
10.11.2022-12.11.2022
Zagreb, Hrvatska