engleski

Effect of lipid raft disruption on AQP3, AQP5, and EFGR pathway

Background: Breast cancer is the leading cause of morbidity and mortality of cancer in women. Therefore, there is a need for understanding modifications in biological processes and signaling pathways that contribute to breast cancer malignancy. Oxidative stress is certainly an important factor that encourages tumor growth by supporting several hallmarks of cancer. Peroxiporins are aquaporins that channel hydrogen peroxide and could, therefore, play a role in regulating intracellular levels of oxidative stress, consequently regulating important signaling pathways. Materials and methods: In this study, the effect of lipid raft disruption on AQP3, AQP5, and EFGR pathway was explored. Lipid rafts were disrupted bycholesterol depletion using methyl-β- cyclodextin. The effect of depletion was measured by MTT and BrdU incorporation assay, and the effects on EGFR signaling pathway was assayed by Western blot levels of pathway components. Results: Our results indicate that EGFR signaling pathway is affected by cholesterol depletion. Conclusions: We have managed to disrupt lipid rafts to interfere with EGFR signaling and are currently studying the consequences on signaling pathway components and levels of aquaporin 3 and aquaporin 5.

aquaporin ; breast cancer ; EGFR ; lipid raft disruption

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