engleski

Effect of lipid raft disruption on NRF2, AQP3, and AQP5 in breast cancer cell lines of different malignancies

Aquaporins are channels that facilitate water transport across the plasma membrane. In addition to water, aquaporins can also transport other small polar molecules, such as glycerol, ions, CO2, urea, and hydrogen peroxide. Transportation of these molecules regulates water balance, cell proliferation, migration, and adhesion. Because of these functions, tumor cells often have higher expression of aquaporins which supports their growth. Out of 13 mammalian aquaporins, six can facilitate hydrogen peroxide and are therefore named peroxiporins. The mere existence of peroxiporins implies their involvement in the regulation of oxidative stress and antioxidative response and could affect the NRF2 expression/activity. In addition to redox signaling pathways, peroxiporins can regulate other signaling pathways such as EGFR. Our aim was to study if lipid raft disruption affects the activity of aquaporins and thus affects NRF2 expression and possibly its translocation to the nucleus. In this study, lipid rafts were disrupted by cholesterol depletion using methyl-β- cyclodextrin. The effect of depletion was measured by MTT and BrdU incorporation assay, and the expression of targeted proteins by Western blot. Depending on the breast cancer cell line, lipid raft disruption influenced AQP3, AQP5, and NRF2 expression differently.

NRF2 ; AQP3 ; AQP5 ; breast cancer

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