engleski

The protective actions of DHEA/S and BDNF against oxidative stress in an in vitro model of vascular dementia

Vascular dementia (VaD), the second most common form of dementia, is generally underrecognized and still poorly understood disease. It develops when the brain´s blood supply is blocked or reduced, causing deprivation of oxygen and nutrients and resulting in damage and death of neurons. The brain is highly susceptible to oxidative stress, due to its richness in fatty acids sensitive to peroxidation, as well as due to high oxygen consumption and free radicals accumulation. Oxidative stress in VaD, characterized by the exacerbated production of reactive oxygen species and insufficient antioxidant defense system, increases neuronal cell abnormalities and triggers apoptosis, leading to cognitive dysfunction and dementia. Neurosteroids dehydroepiandrosterone and dehydroepiandrosterone sulfate (DHEA/S) and neurotrophin brain-derived neurotrophic factor (BDNF) have attracted the attention of researchers investigating their involvement in various brain functions such as neural survival, plasticity, cognition and behavior. The aim of this study was to investigate the protective potential of DHEA/S and BDNF in promoting neuronal survival and preventing oxidative stress caused by brain ischemia, a common characteristic of VaD. Oxygen-glucose deprivation (OGD) was performed in primary mouse neurons derived from C57BL/6 mice, and human SH-SY5Y neuroblastoma cells as in vitro model of VaD. The cells were cultured in glucose- and serum-free medium in a modular incubator chamber filled with N2. Before or after OGD, cells were treated with DHEA/S and BDNF and cell viability and oxidative stress parameters were determined. Our results suggested protective effects of DHEA/S and BDNF against oxidative damage induced by the ischemic injury in neuronal cells.

vascular dementia ; oxidative stress ; DHEA ; DHEAS ; BDNF

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