Beginners guide to sample preparation techniques for transmission electron microscopy (CROSBI ID 328379)
Prilog u časopisu | pregledni rad (znanstveni) | međunarodna recenzija
Podaci o odgovornosti
Dumančić, Ena ; Vojta, Lea ; Fulgosi, Hrvoje
engleski
Beginners guide to sample preparation techniques for transmission electron microscopy
The revolution in microscopy came in 1930 with the invention of electron microscope. Since then, we can study specimens on ultrastructural and even atomic level. Besides transmission electron microscopy (TEM), there are other types of electron microscopes that are not discussed in this review. Here, we have described basic procedures for TEM sample preparation, which include tissue sample preparation, chemical fixation of tissue with fixatives, cryo-fixation performed by quick freezing, dehydration with ethanol, infiltration with transitional solvents, resin embedding and polymerization, processing of polymerized specimens, sectioning of samples with ultramicrotome, positive and negative contrasting of samples, immunolabeling, and imaging. Such collection of methods can be useful for novices in transmission electron microscopy.
TEM; sample preparation; fixation; resin embedding; contrasting; ultramicrotome
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Podaci o izdanju
125 (1-2)
2023.
123-131
objavljeno
0031-5362
1849-0964
10.18054/pb.v125i1-2.25293
Povezanost rada
Biologija, Biotehnologija