engleski

The protective actions of DHEA/S and BDNF in an in vitro model of Parkinson's disease

Introduction: Parkinson's disease (PD) is the second most common neurodegenerative disorder that is characterized by the degeneration of dopaminergic neurons in the substantia nigra. Inducing an in vitro model of PD is a valuable tool for investigating the mechanisms involved in pathogenesis of disease, which is the key to identifying potential therapeutic strategies for PD. Rotenone and 6-hydroxydopamine are neurotoxins commonly used to generate the in vitro model of PD. Dehydroepiandrosterone (DHEA) and its sulfate (DHEAS), and brain-derived neurotrophic factor (BDNF) are involved in neuroprotection and neuroregeneration, while their levels decrease with age and in neurodegenerative diseases such as PD. The aim of this study was to investigate potential neuroprotective actions of these agents in an in vitro model of PD. Materials and methods: Primary mouse neurons derived from C57BL/ 6 mice embryos and rat dopaminergic N27 cell line were injured with rotenone or 6-hydroxdopamine to induce in vitro model of PD. Both cell cultures were treated with DHEA(S) and BDNF, separately and combined, 16 h before injury. Alterations in cell viability were analyzed using the MTT test while oxidative stress parameters were determined using Cellular ROS Assay Kit 2', 7'-dichlorofluorescein diacetate (DCFHDA). Fluorescent dyes Hoechst 33342 and Propidium Iodide were used for staining apoptotic and necrotic cells. Results: Induced injury by rotenone or 6- hydroxydopamine showed lower metabolic activity and higher ROS levels, while DHEA(S), BDNF showed neuroprotective effect separately or combined on both cell cultures. Conclusion: Our results suggest that DHEA(S) and BDNF may play important role in prevention and treatment of PD.

Parkinson disease ; DHEA(S) ; BDNF ; therapy

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