Selected herbicides screened for toxicity and analysed as inhibitors of both cholinesterases (CROSBI ID 325547)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Pehar, Vesna ; Kolić, Dora ; Zandona, Antonio ; Šinko, Goran ; Katalinić, Maja ; Stepanić, Višnja ; Kovarik, Zrinka
engleski
Selected herbicides screened for toxicity and analysed as inhibitors of both cholinesterases
Sets of 346 herbicides in use and 163 no longer in use were collected from open access online sources and compared in silico with cholinesterases inhibitors (ChI) and drugs in terms of physicochemical profile and estimated toxic effects on human health. The screening revealed at least one potential adverse consequence for each herbicide class assigned according to their mode of action on weeds. The classes with most toxic warnings were K1, K3/N, F1 and E. The selection of 11 commercial herbicides for in vitro biological tests on human acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), the enzymes involved in neurotoxicity and detoxification of various xenobiotics, respectively, was based mainly on the structural similarity with inhibitors of cholinesterases. Organophosphate anilofos and oxyacetanilide flufenacet were the most potent inhibitors of AChE (25 μM) and BChE (6.4 μM), respectively. Glyphosate, oxadiazon, tembotrione and terbuthylazine were poor inhibitors with an estimated IC50 above 100 μM, while for glyphosate the IC50 was above 1 mM. Generally, all of the selected herbicides inhibited with a slight preference towards BChE. Cytotoxicity assays showed that anilofos, bensulide, butamifos, piperophos and oxadiazon were cytotoxic for hepatocytes (HepG2) and neuroblastoma cell line (SH-SY5Y). Time-independent cytotoxicity accompanied with induction of reactive oxygen species indicated rapid cell death in few hours. Our results based on in silico and in vitro analyses give insight into the potential toxic outcome of herbicides in use and can be applied in the design of new molecules with a less impact on humans and the environment.
Acetylcholinesterase ; Butamifos ; Cytotoxicity ; Glyphosate ; Neurotoxicity ; Organophosphate pesticides
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Podaci o izdanju
379
2023.
110506
13
objavljeno
0009-2797
1872-7786
10.1016/j.cbi.2023.110506
Povezanost rada
Biologija, Interdisciplinarne prirodne znanosti, Kemija, Poljoprivreda (agronomija)