engleski

Peptidoglycan monomer functionalized gold nanoparticles for L-dopa targeted delivery and probing of their interaction with WGA lectin

The aim of the present study was to synthesize gold nanoparticles (AuNPs-PGM) modified with peptidoglycan monomer (GlcNAc-MurNAc-L-Ala-D- isoGln-mesoDAP(εNH2)-D-Ala-D-Ala) and probe their interaction with model WGA lectin which specifically recognized N-acetylglucosamine (GlcNAc). Our previous study investigates the ability of novel gold nanoparticles to be used as delivery systems for L-DOPA or dopamine by considering the binding capabilities of L-DOPA to the AuNPs-PGM. The results indicate that AuNPs functionalized with PGM enables a significantly higher L-DOPA binding compared to citrate stabilized AuNPs vesicles.1 Now, AuNPs-PGM were treated with the WGA lectin in order to investigate the spatial arrangement of PGM on the AuNPs surface and find out how bound L-DOPA affects the availability of the peptidoglycan and the interaction with lectin. Agglutination is one of the early methods to monitor sugar-receptor interactions, so the WGA lectin is added to the AuNPs-PGM and the hydrodynamic volumes of the resulting aggregates were measured by DLS. Preliminary results demonstrated that WGA lectin successfully recognize and bind to AuNPs-PGM as well as that the particle size grew immediately after WGA lectin is added to the AuNPs-PGM. The increase of size of AuNPs-PGM-L-DOPA was lower in comparison with AuNPs-PGM themselves, but still it is significant. The interaction of lectin and AuNPs-PGM confirmed availability of glycan part of PGM molecule to recognize the lectin receptors on the cell surface and promising potential of prepared nanoparticles to become efficient drug delivery system for Parkinson's disease.

Peptidoglycan monomer ; Gold nanoparticles ; L-dopa ; WGA lectin

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