engleski

Effects of in-feed zeolite treatment on some biomarkers in dairy cows with subclinical mastitis

This study aims to investigate the effect of in-feed clinoptilolite treatment on biomarkers such as serum amyloid A (SAA), haptoglobin, apolipoprotein A–I (APO A–I), and paraoxonase-1 (PON1) activity, triglycerides (TRIG) and high-density lipoprotein cholesterol in dairy cows with subclinical mastitis. A total of 69 Holstein dairy cows, aged 2–7 years, kept on a farm in North- Western Croatia, were included. Cows were randomly assigned into three groups: (1) Clinoptilolite (CPL) group with subclinical mastitis (n = 20), treated in-feed 50 g natural powdered clinoptilolite twice daily from 270th day of pregnancy to the end of study ; (2) nontreated cows (n = 19) with subclinical mastitis (SCM) ; and (3) the control group (CON) of healthy cows (n = 30). Blood samples were taken:on the day of calving and on days 5, 12, 19, 26, 33, 40, and 60 after parturition. The concentration of SAA was highest in the SCM group (p < 0.001). PON1 activity was statistically significantly lower (p < 0.001) in cows from the SCM group than in the CPL and CON groups. Decreased PON1 activity and increased APO A–I were observed in the case of SCM. The positive effect of the addition of vibroactivated clinoptilolite in the feed of treated cows resulted in reduced values of APO A–I, SAA, and haptoglobin, and higher activity of PON1 during the two- month period of lactation after calving, on the basis of which we can assume that they may be less sensitive to infection in SCM.

apolipoprotein A–I ; dairy cows ; haptoglobin ; paraoxonase-1 ; serum amyloid A ; subclinical mastitis

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