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Short-term NaCl intake changes expression of leukocyte integrins in wild type and TFF3-/- knock-out mice

Introduction: Adhesion molecules play an important role in early-phase atherosclerotic changes since they are involved in the mediation of inflammatory cell recruitment as a response to stimuli such as stress following high-salt intake. Methods: Transgenic TFF3-/- knock-out mice (TFF3ko) (N=20) and wild-type mice (WT) (N=20), were each divided into (1) low-salt group (LS) and (2) high-salt group (HS). For one week (7 days), 10-week-old animals were fed with standard rodent chow (0.4% NaCl) (LS) or food containing 4% NaCl (HS). Whole blood samples and mesenteric lymph nodes (MLNs) were collected. C-reactive protein (CRP) was measured via Luminex assay from serum samples. Measurement of integrins expression was performed via flow cytometry. Results: There was a significant increase of CRP only in WT mice following the HS diet, while there were no changes in the TFF3ko group. CD49d/VLA-4 expression decreased on peripheral blood leukocytes (PBL) in both groups following the HS diet. CD11a/LFA-1 expression significantly increased only on peripheral blood Ly6C-CD11ahigh monocytes in WT mice following salt-loading. Discussion: HS diet activated PBLs leading to adhesion/migration only in the WT group due to increased LFA-1 expression which was a result of the interaction between genotype and treatment. A decrease in VLA-4 expression in both groups was a result of genotype or treatment alone. Conclusion: HS diet produced a lower level of inflammation in TFF3ko mice due to gene depletion.

inflammation, integrins, knock-out, sodium chloride, trefoil factor-3

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