engleski

Optimization of the biocatalytic cascade synthesis of (R)-3-hydroxy-γ-butyrolactone by means of kinetic modelling

Biocatalysis can be defined as the use of enzymes or whole cells to speed up synthesis reactions. [1] The newer concept in biocatalysis is the synthesis of valuable chemicals achieved through complex cascades with different enzymes sustainably and efficiently. In comparison with conventional chemical processes, cascade reactions offer many advantages like no need for isolation of intermediate products, mild and controlled reaction conditions, simple and economically profitable reaction system which is composed of one reactor. [2] 3-hydroxy-γ-butyrolactone (HBL) is a functional chiral building block with application in the synthesis of different polymers, solvents and pharmaceuticals. With an enzymatic approach, the synthesis of HBL can be economically viable while maintaining the high selectivity of this valuable chemical. [3] In this work, the kinetic model for the cascade reaction (Fig. 1) was developed. Model validation was done by performing the batch reactor experiments. Simulations were used to optimize reaction conditions and choose the best reactor design. References [1] Đ. Vasić-Rački, Z. Findrik, A. Vrsalović Presečki, Appl. Microbiol. Biotechnol. 91 (2011), 845–856. [2] M. Sudar, Z. F. Blažević, Enzyme Cascade Kinetic Modelling, in: S. Kara, F. Rudroff Enzyme Cascade Design and Modelling, Springer International Publishing, Cham, 2021, pp. 91–108. [3] H. Dhamankar, Y. Tarasova, C. H. Martin, K. L. J. Prather, Metab. Eng. 25 (2014) 72-81.

biocatalysis ; cascade reaction ; HBL ; kinetic modelling

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