Cytokine and chemokine levels in the cerebrospinal fluid and plasma samples of mild cognitive impairment and Alzheimer's disease subjects (CROSBI ID 734481)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Španić Popovački, Ena ; Babić Leko, Mirjana ; Brgić, Klara ; Vogrinc, Željka ; Boban, Marina ; Klepac, Nataša ; Borovečki, Fran ; Šimić, Goran
engleski
Cytokine and chemokine levels in the cerebrospinal fluid and plasma samples of mild cognitive impairment and Alzheimer's disease subjects
In addition to amyloid β and tau protein pathology, Alzheimer's disease (AD) is characterized by dysregulation of the inflammatory response. The main objective of this study was to find out how the concentrations of immune mediators (cytokines and chemokines) differ between AD, patients with mild cognitive impairment (MCI), and healthy controls (HC) in two different types of samples, cerebrospinal fluid (CSF) and plasma. We also wanted to determine the cytokine profile for each group. Using the Bio-Plex Pro Human Cytokine 48-Plex Screening Panel, we determined the levels of 35 cytokines in CSF and 47 in plasma samples. Twenty-nine AD, 35 MCI, and 40 HC CSF and 102 AD, 37 MCI, and 10 HC plasma samples were analyzed. In the plasma samples, only 4 cytokines differed significantly between the groups (CTACK, IL-12 (p40), IL-4, IL1Rα), whereas analysis of the CSF samples revealed significantly different levels of 15 immune mediators (IL-1β, IL-1α, IL-3, IL-4, IL-7, IL-16, IL-17A, IL-18, IFN-γ, GRO-α, MIP-1β, CTACK, TNF-α, IL-2Rα, IP-10). Most of the mediators whose levels differed significantly were increased in the AD group ; only IP-10 had the highest levels in the MCI group. CSF analysis showed that the AD group was characterized by a pro-inflammatory profile and a higher rate of an adaptive immune response. Higher levels of predominantly pro-inflammatory cytokines (IL-1β, IL-18, MIG, EOTAXIN, MIP-1α, MIP-1β, TNF-β) were associated with higher levels of cognitive deficits. This study has shown that measurement of immune mediators in CSF, but not in plasma, may be a good strategy to track the immune response during disease progression and could be a promising tool for evaluating potential future immune and anti-inflammatory interventions.
Alzheimer’s disease ; inflammation ; immune mediators ; cerebrospinal fluid, multiplex ELISA
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
75-75.
2023.
nije evidentirano
objavljeno
Podaci o matičnoj publikaciji
Neurologia Croatica. Supplement
Šimić, Goran ; Mimica, Ninoslav
Zagreb: Denona
1331-5196
Podaci o skupu
2nd Congress of the Croatian Alzheimer Alliance
poster
29.03.2023-01.04.2023
Varaždin, Hrvatska