engleski

Interaction between 4-hydroxynonenal and IGF-1 on human osteosarcoma cells

4-Hydroxynonenal (HNE), the end product of lipid peroxidation, is not only toxic, but also growth modulating factor, influencing signal transduction, gene expression, cell proliferation and differentiation. These processes are also controlled by different cytokines and growth factors. Insulin-like growth factor, IGF-1, is synthesized by osteoblasts and has important role in osteoblast proliferation and differentiation as well as bone growth and development. The purpose of our study was to see the interference between HNE and IGF-1 and their influence on proliferation of osteoblast-like HOS cells and influence of HNE on IGF-1 synthesis in HOS cells. Experiments showed that HNE interfered with the effects of IGF-1 on cell proliferation and caused dose-dependent decrease in IGF-1 production in HOS cells. As IGF-1 is important autocrine and paracrine growth regulator for mesenchimal cell, we conclude that HNE can modulate its effects on osteoblasts, probably also influencing bone growth and development in vivo. Effects of IGF-1 on cells exposed to HNE can be relevant for protecting cells from harmful oxidative stress.

4-Hydroxynonenal; lipid peroxidation; insulin-like growth factor; human osteosarcoma cells

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