engleski

DIPEPTIDYL-PEPTIDASE IV (DPP IV/CD26) AFFECTS THE WOUND HEALING PROCESS IN A STREPTOZOTOCIN-INDUCED DIABETES MODEL IN MICE

INTRODUCTION: Diabetes mellitus is a metabolic disorder with multiple etiology, characterised by chronic hyperglycemia with disturbances of metabolism of carbohydrates, fats and proteins. It results from defects in insulin secretion, action or both. Dipeptidyl-peptidase IV/CD26 (DPP IV/CD26) molecule is known to be involved in an array of physiological and pathological processes, in the regulation of glycaemia as well. A variety of complications caused by diabetes can cause hospitalizations as infections and gangrene. DPP IV/CD26 inhibitors such as alogliptin, linagliptin and saxagliptin can be used to treat diabetes mellitus. The aim of this reseaarch was to investigate the processes of wound healing in conditions of CD26 deficiency in experimental hyperglycaemia. MATERIALS AND METHODS: In this experiment two strains of mice were used, wild type and CD26- deficient mice. Experimental diabetes was induced using a solution of streptozotocine in citrate buffer (50mg/kg, i.p.) during five days. Serm samples as well as pancreas, cutaneous (control) tissue and wound tissue were analyzed. Pathohistological, immunohistochemical and immunobiochemical analyses were performed on wound samples and control skin. Serum samples were analysed for DPP IV/CD26 activity and concentration of target angiogenic factors. RESULTS: Results of this study revealed that DPP IV/CD26 has an important role in the regulation of blood glucose concentration. Inactivation of DPP IV/CD26 improves the state associated with hyperglycemia. The process of cutaneous wound healing is improved in conditions of CD26 deficiency. CONCLUSION: The inhibition of DPP IV/CD26 has positive effects on the wound healing process in hyperglycemia. DPP IV/CD26 inhibition can be suggested as a therapeutic option for treatment of diabetes mellitus and its complications like delayed wound healing.

diabetes mellitus, DPP IV/CD26, hypergylicemia, wound healing

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