engleski

Wound Healing in Dipeptidyl-peptidase IV (DPP IV/CD26) Deficient Diabetic Mice

Introduction: Dipeptidyl-peptidase IV/CD26 molecule (DPP IV/CD26) is widely known, except for its involvement in a multitude of physiological and pathological processes, for the role in regulation of glycaemia. Complications caused by diabetes such as infections, ulcerations and gangrene are the main causes for hospitalization. DPP IV/CD26 inhibitors are available as a treatment option for patients with diabetes type II. Inhibition of DPP IV/CD26 accelerates healing of chronic diabetic ulcerations by induction of a histological pattern consistent with enhanced angiogenesis. We hypothesized a significant role of DPP IV/CD in mechanisms of cutaneous wound healing in hyperglycemia. Our aim was to research the process of wound healing in conditions of CD26 deficiency in experimental hyperglycemia in order to acquire more insights on the role of DPP IV/CD26 in cutaneous reparation and regeneration. Materials and methods: A streptozotocin-model of diabetes was induced in wild-type mice and CD26 deficient type. Experimental wounds were performed on mice dorsal regions and animals were sacrificed on determined time schedule. Pathohistological, histomorphometrical, immunohistochemical and immunobiochemical analyses were performed on wound samples and control skin while serum samples were analysed for DPP IV/CD26 activity and concentration of target angiogenic factors. Results: Our hypothesis was confirmed by this study since we shown that DPP IV/CD26 plays an important role in the regulation of blood glucose concentration and its inactivation improves state associated with hyperglycemia. Moreover, the process of cutaneous wound healing is improved in conditions of DPP IV/CD26 deficiency with elevated local expression of proangiogenic factors. Conclusion: Inhibition of DPP IV/CD26 has beneficial effects on the wound healing process in hyperglycemia, which supports the significance of DPP IV/CD26 inhibition as a therapeutic option for the treatment of diabetes.

Diabetes Mellitus ; DPP IV/CD26 ; Hyperglycemia

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