engleski

1500-kcal-nutritionally balanced reduction vs. 567-kcal-hospitally controlled diet differences on anthropometric, biochemical, oxidative, primary and permanent DNA damage parameters in severely obese patients with BMI ≥ 35kgm-2 after 3 weeks of diet–preliminary results

We wanted to check the differences of anthropometric, biochemical, oxidative, primary and permanent DNA damage parameters in severely obese patients with BMI ≥ 35kgm-2 after 3 weeks between 567 kcalhospitaly- controlled VLCD (26 patients) and nutritionally balanced reduction diet of 1500 kcal daily (10 patients in this preliminary study). Both diets did not cause significant differences in triglycerides, TSH, ft4 and ft3 levels, but both caused decrease in anthropometric and other biochemical parameters. Among anthropometric parameters only VLCD group had significant decrease in weight. Diets caused BMI reduction (VLCD-in average 3-4 BMI units’ loss, 1500-kcal-1 unit), excessive weight loss (between 10-35%-VLCD and 13%-1500 kcal) and weight loss (average 9 kg, range 4.8-14.4kg for VLCD and only 4 kg average for 1500 kcal). Also, only VLCD caused significant decrease in glucosis, urea, total cholesterol-C, HDL-C, LDL-C, insulin and HOMA-IR levels, while 1500-kcal caused only slight decrease. Changes were also observed in DNA damage in the following manner: VLCD caused significant decrease in TI comet assay values, while both diet caused significant decrease in Net FPG TI values. In cytochalasin-B-blocked micronucleus assay, VLCD caused significant decrease of MN (micronuclei), NB (nuclear buds), NPB (nucleoplasmic bridges) and apoptosis frequencies, while 1500 kcal caused also decrease, but only significant one was in the frequency of NB and apoptosis. According to this preliminary results it seems that more strict diet has better results on human health and DNA stability improvement in severely obese patients with comorbidities, but both were able to decrease oxidative DNA damage, that is considered as the first factor leading to increased genomic instability, that again can lead to higher cancer risk and mortality.

Obesity, VLCD, DNA damage

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