engleski

Influence of the 3-weeks-1500-kcal-well-balanced- Diet on DNA Damage (in)Stability, Anthropometric and Biochemical Parameters in Normotensive and Hypertensive Obese Group with BMI ≥35 kg/m2- a Pilot Study

Background: DNA damage induced by reactive oxygen species (ROS) occurs more often in hypertensive than in normotensive individuals, and more in obese individuals. Besides genetic and lifestyle factors, diet can have a great impact on hypertension and obesity development, and a proper restricted diet should have an impact on hypertension and obesity regulation. Objective: The study checked for the improvements in primary, oxidative, permanent DNA damage stability, anthropometric and biochemical parameters in obese people with body mass index (BMI) ≥35 kg/m2 who were with medically controlled hypertension and without hypertension after the 3 weeks of diet. Methods: In 27 obese with BMI≥35 kg/m2 (13 normotensive, 14 with medically controlled hypertension), who agreed to be on a 3-weeks-1500- kcal-nutritionally-balanced diet, anthropometric (InBody 270), biochemical (Beckman Coulter 480 and Sysmex XS-1000i), primary and oxidative (alkaline comet assay-SCGE, -/FPG), and permanent DNA damage (micronucleus cytome assay-CBMN) parameters were analyzed before and after the diet and two groups were compared (TIBCO Software Inc-14.0.0.15). Results: In hypertension medically controlled group, there was no improvement SCGE, but oxidative DNA damage decreased. CBMN demonstrated an improvement in mitotic index, and a decrease in permanent DNA damage parameters, apoptosis and necrosis. Well-balanced diet managed to increase the vitamins´ levels and decrease glucose, leukocytes, urea, GGT, cholesterol and triglycerides levels. Conclusion: In a pilot study of obese hypertensive patients on a 3-weeks-1500-kcal-standard-well- balanced-reduction diet, results demonstrated that diet helped to reduce weight, BMI, glucose, oxidative and permanent DNA damage levels. A larger group of volunteers would give better insight into whether short-term reduction well- balanced diet can have an impact on health stabilization in obese hypertension individuals.

Obesity, hypertension, DNA damage

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