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MiR-182-5p and MiR-375-3p in blood plasma as biomarkers for prostate cancer

With prostate cancer (PCa) being the most commonly diagnosed neoplasm among men and often resembling benign prostate hyperplasia (BPH), biomarkers with a higher differential value than PSA are required. We investigated the expression of certain miRNAs from liquid biopsies as potential epigenetic biomarkers of PCa. The absolute expression of miR- 375-3p and miR-182-5p were quantified in blood plasma and seminal plasma of 65 PCa and 58 BPH patients by digital droplet PCR. The sensitivity and specificity of these microRNAs were determined using ROC curve analysis. The higher expression of miR-182-5p and miR-375- 3p in the blood plasma of PCa patients was statistically significant as compared to BPH (p = 0.0363 and 0.0226, respectively). Their combination achieved a specificity of 90.2 % for predicting positive or negative biopsy results, while PSA cut-off of 4 µg/L performed with only 1.7 % specificity. In seminal plasma, miR-375- 3p, and miR-182-5p showed a statistically significantly higher expression in PCa patients with PSA >10 µg/L compared to ones with PSA >10 µg/L. MiR-182-5p and miR-375-3p in blood plasma show higher performance than PSA in differentiating PCa from BPH. Seminal plasma requires further investigation as it represents an obvious source for PCa biomarker identification.

miRNA ; prostate cancer ; liquid biopsy ; biomarkers

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