engleski

Yeast cell wall protein Scw10 proteolytic processing

The Scw4, Scw10, and Scw11 proteins form a group of putative glucanases located in the yeast Saccharomyces cerevisiae cell wall. In the previously published study, Scw10 protein was shown to be up to 10-fold more abundant in several clinical isolates compared with laboratory yeast strain, suggesting its possible role in yeast virulence. Its paralog Scw4 undergoes complex proteolytic processing. Scw4 is processed by the Kex2 protease and subsequently by yapsin proteases, which affects the biological activity of Scw4 and its ability to bind to cell walls. The proteolytic processing or binding of Scw10 has not been thoroughly investigated. It has also been shown that both Scw4 and Scw10 can form non- covalent and covalent bonds with the wall that differ from previously known protein-wall bonds. Although these bonds resemble those of Pir proteins, both Scw4 and Scw10 lack the typical Pir binding sequence. In this study, the processing of Scw10 and its potential impact on its biological function are investigated. In addition, the role of several putative Scw4/Scw10 binding sequences in the formation of a covalent bond to cell wall structures is explored.

Scw10, yeast, cell wall

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