engleski

UEG13-ABS-4931 BPC 157 FISTULA-HEALING EFFECT CLOSES TRACHEOTOMY WOUND IN RATS

INTRODUCTION: BPC 157 is an original anti-ulcer peptide (GEPPPGKPADDAGLV, M.W. 1419) stable in human gastric juice more than 24 hours, successful in trials for inflammatory bowel disease, phase II, wound treatment, no toxicity or side effects were reported, LD1 was not achieved, effective alone without carrier. BPC 157 also closes gastrocutaneous, colocutaneous and esophagocutaneous fistulas. Thereby, we investigate whether BPC 157-fistulas healing may be relevant in tracheotomy wound healing. AIMS&METHODS: Rats were underwent to standard horizontal tracheotomy, trachea opened horizontally, no cannula was inserted and edges of the trachea were sutured on to a skin. BPC 157 (10 μg/kg, 10 ng/kg) was applied (i) in drinking water until the animals were euthanized, or (ii) once a day intraperitoneally (first application 30 min following surgery, last 24 h before euthanization, at 3rd postoperative day. With 3 mm diameters, trachea was opened on the opposite side of the tracheotomy wound and inner part of the wound was also measured. RESULTS: BPC 157 administered parenterally or perorally acceleratedhealing of tracheotomy wound and showed functional, macroscopic and histological healing improvements. In average, with respect to initial size, both outer and inner part of the tracheotomy wound were improved: i.e., 10 ug/kgof BPC 157 in drinking water improved healing by 40 - 100% for the outer part (skin), and by 60-100% for the inner part (trachea) of the wound. Contrary, controls showed only poor by 0-37.5% for the outer part (skin), and by 0-50% for the inner part (trachea) of the tracheotomy wound (Fig. 1). Further, animals treated with BPC 157 showed slower weight loss than the control. CONCLUSION: BPC 157 fistula-healing effect may close tracheotomy wound.

BPC 157, fistula, healing, rats, tracheotomy

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