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Su1209 Protective and Reparative Effect of Petadecapeptide BPC 157 on Mice Blood, Liver and Kidney Cells

Su1209 Protective and Reparative Effect of Petadecapeptide BPC 157 on Mice Blood, Liver and Kidney Cells Ivan Sopf, Nevenka Kopjar, Mirta Milic, Damir Sirovina, Sandro Mise, Tomislav Becejac, Domagoj Drmic, Nada Orsolic, Sven Seiwerth, Predrag Sikiric Aim. We investigated the protective and reparative effect of BPC 157 on blood, liver and kidney cells of mouse, after cisplatin-caused DNA damage. During the trial the mice were exposed to the effects of BPC 157 and cisplatin cytostatic with the aim to assess a protective effect of BPC 157 on healthy cells. Methods. The mice were divided into 4 groups: control ; BPC 157 (intraperitoneally (ip) 100 μg kg-1 for 3 days) ; BPC 157 and cisplatin ; cisplatin (intraperitoneally (ip) 10 mg kg-1 applied once third day before animals were sacrified.We monitored the damage in blood, liver and kidney DNA cells after 0, 1, 6, and 24 hours and the speed of DNA repair using comet assay and micronuclei test. Results: The comet test results have shown that the DNA was most damaged by cisplatin and the highest values of tail length were found 1 hour after exposure (24% lymphocytes ; 14.2% kidney cells ; 12.2% liver cells). The largest number of apoptotic cells was found in kidney cells . The joint administration of BPC 157 and cisplatin resulted in less damage incurred by cisplatin (lymphocytes damage after 1 hour 2.9% vs cisplatin 24.4% ; kidney damage 0.3% vs 14.2% cisplatin ; liver damage 2.8% vs 12.2% cisplatin). A number of micronuclei in the cisplatin group increases proportionally to the period of exposure and has the highest value after 24 hours (55.43±8.10 ; min=35, max=90). The micronuclei values in BPC 157 are equal to the control group. It has been demonstrated that the joint effect of BPC and cisplatin decreases the micronuclei count after 24 hours (28.50±6.94 ; min=7, max=53). It was shown that the most sensitive organ to the toxic effects of cisplatin was the kidney followed by liver and lymphocytes. Conclusion: Results indicated that BPC 157 has a protective effect on healthy cells ; probably the protective effect of BPC 157 is based on a reduced intake of cisplatin into the cell or its participation in the process of DNA-reparation of damaged cells, which requires further research. 1.Fenech M. Cytokinesis-block micronucleus assay evolves into a "cytome" assay of chromosomal instability, mitotic dysfunction and cell death. Mutat Res 2006 ; 600(1-2):58-66, 2. Lu Y, Cederbaum AI. Cisplatin-induced hepatotoxicity is enhanced by elevated expression of cytochrome P450 2E1. Toxicol Sci 2006 ; 89:515- 523.

Pentadecapeptide BPC 157, Mice Blood, Liver, Kidney Cells

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